Congenital toxoplasmosis is an important cause of miscarriage worldwide. There is high seropositivity of T. gondii among pregnant women in Bangladesh. The aims of the study were to estimate the presence of T. gondii in fetoplacental tissue among women with miscarriage and assess risk factors for toxoplasma infection in pregnant women. This cross-sectional study was performed for a period of one year on 138 patients. Obstetric parameters; cat, food, and soil related risk factors for toxoplasmosis were recorded and PCR analysis of fetoplacental tissue was performed for B1 and GRA6 gene in fetoplacental tissue by conventional PCR analysis. Comparisons were made between PCR positive and negative patients with regards to presence of risk factors for toxoplasmosis using Chi-square test or Fisher’s exact for categorical data; and t test or Mann Whitney U test for continuous data where appropriate. Among a total of 138 patients with miscarriage, 38 patients’ fetoplacental tissue (27.5%) were found to be positive for T. gondii. Age ranged from 18 to 37 years, mean 25 (±5) years. Living in a neighborhood with a cat was the only significant risk factor associated with PCR positivity (P=0.03). Other cat related factors and exposure to other animals were not related to PCR positivity. T. gondii was present in about one-fourth women with miscarriage at a tertiary hospital which demands further research.
| Published in | Journal of Gynecology and Obstetrics (Volume 10, Issue 4) |
| DOI | 10.11648/j.jgo.20221004.16 |
| Page(s) | 196-202 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2024. Published by Science Publishing Group |
Fetoplacental, Miscarriage, Molecular Diagnostic Technique, Toxoplasmosis
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APA Style
Tamanna Ferdous Reza, Shahanara Chowdhury, Nasreen Banu, Abu Mohammad Abdul Momen Zonaed Siddiki. (2022). Molecular Diagnosis of Toxoplasma Gondii in Fetoplacental Tissue of Women with Miscarriage and Assessment of Risk Factors for Toxoplasmosis. Journal of Gynecology and Obstetrics, 10(4), 196-202. https://doi.org/10.11648/j.jgo.20221004.16
ACS Style
Tamanna Ferdous Reza; Shahanara Chowdhury; Nasreen Banu; Abu Mohammad Abdul Momen Zonaed Siddiki. Molecular Diagnosis of Toxoplasma Gondii in Fetoplacental Tissue of Women with Miscarriage and Assessment of Risk Factors for Toxoplasmosis. J. Gynecol. Obstet. 2022, 10(4), 196-202. doi: 10.11648/j.jgo.20221004.16
AMA Style
Tamanna Ferdous Reza, Shahanara Chowdhury, Nasreen Banu, Abu Mohammad Abdul Momen Zonaed Siddiki. Molecular Diagnosis of Toxoplasma Gondii in Fetoplacental Tissue of Women with Miscarriage and Assessment of Risk Factors for Toxoplasmosis. J Gynecol Obstet. 2022;10(4):196-202. doi: 10.11648/j.jgo.20221004.16
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Download@article{10.11648/j.jgo.20221004.16,
author = {Tamanna Ferdous Reza and Shahanara Chowdhury and Nasreen Banu and Abu Mohammad Abdul Momen Zonaed Siddiki},
title = {Molecular Diagnosis of Toxoplasma Gondii in Fetoplacental Tissue of Women with Miscarriage and Assessment of Risk Factors for Toxoplasmosis},
journal = {Journal of Gynecology and Obstetrics},
volume = {10},
number = {4},
pages = {196-202},
doi = {10.11648/j.jgo.20221004.16},
url = {https://doi.org/10.11648/j.jgo.20221004.16},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jgo.20221004.16},
abstract = {Congenital toxoplasmosis is an important cause of miscarriage worldwide. There is high seropositivity of T. gondii among pregnant women in Bangladesh. The aims of the study were to estimate the presence of T. gondii in fetoplacental tissue among women with miscarriage and assess risk factors for toxoplasma infection in pregnant women. This cross-sectional study was performed for a period of one year on 138 patients. Obstetric parameters; cat, food, and soil related risk factors for toxoplasmosis were recorded and PCR analysis of fetoplacental tissue was performed for B1 and GRA6 gene in fetoplacental tissue by conventional PCR analysis. Comparisons were made between PCR positive and negative patients with regards to presence of risk factors for toxoplasmosis using Chi-square test or Fisher’s exact for categorical data; and t test or Mann Whitney U test for continuous data where appropriate. Among a total of 138 patients with miscarriage, 38 patients’ fetoplacental tissue (27.5%) were found to be positive for T. gondii. Age ranged from 18 to 37 years, mean 25 (±5) years. Living in a neighborhood with a cat was the only significant risk factor associated with PCR positivity (P=0.03). Other cat related factors and exposure to other animals were not related to PCR positivity. T. gondii was present in about one-fourth women with miscarriage at a tertiary hospital which demands further research.},
year = {2022}
}
TY - JOUR T1 - Molecular Diagnosis of Toxoplasma Gondii in Fetoplacental Tissue of Women with Miscarriage and Assessment of Risk Factors for Toxoplasmosis AU - Tamanna Ferdous Reza AU - Shahanara Chowdhury AU - Nasreen Banu AU - Abu Mohammad Abdul Momen Zonaed Siddiki Y1 - 2022/08/04 PY - 2022 N1 - https://doi.org/10.11648/j.jgo.20221004.16 DO - 10.11648/j.jgo.20221004.16 T2 - Journal of Gynecology and Obstetrics JF - Journal of Gynecology and Obstetrics JO - Journal of Gynecology and Obstetrics SP - 196 EP - 202 PB - Science Publishing Group SN - 2376-7820 UR - https://doi.org/10.11648/j.jgo.20221004.16 AB - Congenital toxoplasmosis is an important cause of miscarriage worldwide. There is high seropositivity of T. gondii among pregnant women in Bangladesh. The aims of the study were to estimate the presence of T. gondii in fetoplacental tissue among women with miscarriage and assess risk factors for toxoplasma infection in pregnant women. This cross-sectional study was performed for a period of one year on 138 patients. Obstetric parameters; cat, food, and soil related risk factors for toxoplasmosis were recorded and PCR analysis of fetoplacental tissue was performed for B1 and GRA6 gene in fetoplacental tissue by conventional PCR analysis. Comparisons were made between PCR positive and negative patients with regards to presence of risk factors for toxoplasmosis using Chi-square test or Fisher’s exact for categorical data; and t test or Mann Whitney U test for continuous data where appropriate. Among a total of 138 patients with miscarriage, 38 patients’ fetoplacental tissue (27.5%) were found to be positive for T. gondii. Age ranged from 18 to 37 years, mean 25 (±5) years. Living in a neighborhood with a cat was the only significant risk factor associated with PCR positivity (P=0.03). Other cat related factors and exposure to other animals were not related to PCR positivity. T. gondii was present in about one-fourth women with miscarriage at a tertiary hospital which demands further research. VL - 10 IS - 4 ER -
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