Volume 8, Issue 3, May 2020, Page: 51-54
The Effect of Paclitaxel on the Viability of U14 Cells
Gao Xuesong, Department of Obstetrics and Gynecology, The First Affiliated Hospital of Jinan University, Guangzhou, China
Lin Shaoqiang, The Hospital Management Department of Guangdong Pharmaceutical University, Guangzhou, China
Wang Xiaoyu, Department of Obstetrics and Gynecology, The First Affiliated Hospital of Jinan University, Guangzhou, China
Received: Apr. 18, 2020;       Accepted: Apr. 29, 2020;       Published: May 11, 2020
DOI: 10.11648/j.jgo.20200803.11      View  19      Downloads  10
Abstract
The research is aimed to study the effect of paclitaxel on the viability of U14 cell line of cervical cancer, and to provide new ideas for further exploring the mechanism of paclitaxel chemotherapy to cervical cancer. Then, different concentrations of paclitaxel were used to treat U14 cells of cervical cancer in logarithmic phase growth. After culturing these cells for 24h, 48h or 72h, MTT method and automatic enzyme-linked immunosorbent assay system were used to evaluate the survival rate of cultured cells with different concentrations of paclitaxel. These results showed that paclitaxel has a significant inhibitory effect on the proliferation of U14 cells in a concentration- and time-dependent manner, compared with the control group. Paclitaxel concentration of the IC50 of U14 cells was 168.8μg/ml at 24h, 22. 15 μg/ml at 48h and 8. 04μg/ml at 72 h. As the time increased, the IC50 value of U14 cells gradually decreased. The results of linear regression analysis are as follows: 24 h, y=-0.005x + 1.344, R2=0.779; 48 h, y=-0.013x +0.788, R2=0.923; 72 h, y=-0.056x + 0.950, R2=0.908. The dose-effect curve of paclitaxel on U14 cells for 48 h and the trend of linear regression fitting are better, and the growth inhibition of cells shows a clear relationship between time and dose. In conclusion, paclitaxel has an obvious inhibitory effect on the activity of cervical cancer U14 cells in mice, which provides new ideas for further exploring the mechanism of cervical cancer paclitaxel chemotherapy.
Keywords
Paclitaxel, Cervical Cancer, Cell Viability, U14 Cell
To cite this article
Gao Xuesong, Lin Shaoqiang, Wang Xiaoyu, The Effect of Paclitaxel on the Viability of U14 Cells, Journal of Gynecology and Obstetrics. Vol. 8, No. 3, 2020, pp. 51-54. doi: 10.11648/j.jgo.20200803.11
Copyright
Copyright © 2020 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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